Buffer ditharollo: ho lokisetsa le tshebediso

Buffers - e ditharollo nang tsamaiso e khopo buffering. tsamaiso ea Buffer bitsoa motsoako oo ho hlophisoa e na le e qoolloa ditirisanommogo karo-karolelano acid fokolang le letsoai ea bona le makalana matla kapa makalana fokolang le matsoai a bona ka acid e matla. ditharollo joalo ba le mahloriso a tsitsitseng ea H + ions ho se nke lehlakore diluting solvent (metsi) le phaella ka ho re ho bona ka chelete e itseng ea acid le matla kapa le bokatlase.

Buffer ditharollo di metsi a maoatle, ditharollo mobu le ka lintho tse phelang. tsamaiso ea tsena sebetsa laola mesebetsi e tšehetsang a sebetsang a itsoara joang ka seaplane a sa le boleng boletsweng hlokahalang bakeng sa ketsahalo e le atlehileng. a itšoare tshilong ya dijo. ditharollo Buffer ba classified ka asiti le tsa motheo. Mohlala e ka ba pele ba ea acetate pafara tsamaiso, ea bobeli e - ammonium. Khetholla buffers tlhaho le maiketsetso. A tsa tlhaho pafara tharollo ke mali e nang le hydrocarbonate, phosphate, protheine, haemoglobin le asiti oona tsamaisong pafara. Maiketsetso pafara ka 'na ba ea acetate pafara, e bopilweng ka CH3COOH.

Features ya sebopeho hare le mochine oa bohato ba litsamaiso pafara re tšohleng mohlala oa tsamaiso ea acetate pafara: ea acetate acid e / sodium ea acetate. Ka seaplane ea metsi e pafara oona tsamaisong dikarolo di tlas'a electrolytic dissociation. Sodium ea acetate e le letsoai ea acid e ka fokola 'me la botlaaseng matla ka ho feletseng dissociates ka ions. Ho ba teng ha anions ka motsoako pafara itšetlehile ka ea mahloriso ea letsoai ho eona 'me lengolo la sona la dissociation. Ea mahloriso ea H + ions tsamaisong ea pafara ke ka ho toba tekanyo e lekanang ya ho ea mahloriso ea acid e ka ho le inversely tekanyo e lekanang ya ho dikahare tsa lona tsa letsoai ea acid e ena.

Kahoo, ea mahloriso ea H + ions ka pafara mantlha ke ho toba tekanyo e lekanang ya ho ea mahloriso ea letsoai ho sona 'me inversely tekanyo e lekanang ya ho ea mahloriso ya botlaaseng itseng.

Ikatisetsa laboratori ea, sebelisa ditharollo pafara le makgabane a tsebahalang ea ph. Kahoo, ba lokisetsa ho ea ditharollo pafara e etsoa ka ho sebelisa ditharollo tsa acid e ka fokola 'me letsoai lona le botlaaseng matla kapa fokolang botlaaseng le letsoai lona le asiti e matla. Ebe ka ho fetola karo-karolelano ditirisanommogo ea likarolo, ho pafara ditharollo etsoa le setpoint ph. Ba bang ba ipotsa hore na ba lokisetsa ho ea tharollo.

Ka mohlala, e ke ho hlokahala hore ho lokisetsa ea acetate pafara ka melao ea boitšoaro e 'maloa ea ph. Qalong lokisetsa 5M tharollo ea acid e ea acetate le sodium ea acetate. Bakeng sa ba lokisetsa ho ea ka tharollo pele nka 50 di ml ka mong oa metsoako eo. Ho Tataisoa ke moralo wa, ea mahloriso ea H + ions ka tharollo sephetho.

Bakeng sa latelang pafara tharollo nka 80 di ml ea tharollo acid e le 20 di ml tse tharollo letsoai lokisetsa pele. Ho na le a 'maloa a formulations tsa ditharollo tse fapa-fapaneng pafara sebelisoa ka and analysis lik'hemik'hale le ka go ikatisetsa laboratoring.

Bakeng sa e tloaelehileng ea thepa buffers. Ho ba haholo-holo o re Buffering - le matla a ho boloka tiisetso ea mahloriso ea H + ions ha eketsa ka palo e itseng ea pafara tharollo ea acid e ka matla kapa botlaaseng matla. Ka mohlala, haeba e ea acetate pafara ho eketsa e nyenyane ea chloride acid e, ph battlefield ho lehlakoreng acid e ha e etsahala, ho tloha chloride acid e tla itšoara joang phapanyetsano li bole kapele le letsoai ea acid e ka fokola. Itsoara joang ka tsa matla acid e nang le bokhoni tseleng ph ho lehlakoreng acid e, 'me nkeloa sebaka ke ho se nke lehlakore fokolang acid e letsoai. Tekanyo ea ho dissociation la ho fokola electrolyte tharollo le e ntseng e eketseha ea mahloriso decreases ho lefela, le ph battlefield se hlahile.